Difficulty providing a saliva sample for DNA testing

Difficulty producing enough saliva If you can’t produce enough saliva in one try, deposit a little saliva into the tube and refrigerate it until you can add more. Repeat until saliva reaches the line on the tube. The laboratory does not test blood or hair samples.

How do you get enough saliva for DNA testing?

If providing adequate sample volume is a challenge for you or someone you are assisting, the following may help increase saliva production:

  1. Gently rubbing the outside of your cheeks.
  2. Making chewing motions with your mouth.
  3. Smelling or imagining sour foods such as lemons.
  4. Thinking about your own favorite food.

How do you get a good saliva sample?

Do NOT eat, drink, smoke or chew gum for 30 minutes before giving your saliva sample. Do NOT remove the plastic film from the funnel lid that contains the clear liquid. Spit into the open funnel until the amount of saliva (not bubbles) reaches the fill line. Most people take 2 to 5 minutes to fill the tube.

Why does my saliva not have enough DNA?

Firstly, the saliva sample may have been compromised, either by the collection tube leaking in transit or by a failure of the preservative solution to mix with the saliva after collection. Secondly, the saliva may not contain enough useful DNA (a point I’ll return to below), or the DNA may be too degraded to use.

How can I get saliva without bubbles?

If you find yourself having difficulty making enough saliva try these:

  1. Gently massage your cheeks.
  2. Gently gnaw or chew on your tongue.
  3. Think about something sour like your favorite sour candy or lemons.
  4. Think about what you ate last or are going to eat next; imagine the smells, tastes and textures.

Why does my AncestryDNA test keep failing?

The most common reason that a 23andMe, or any other DNA test, could fail would be that your sample did not contain enough DNA to be extracted for reliable results. Our saliva contains DNA not only from epithelial cells, but from white blood cells, too.

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